Isolation of single yeast cells by optical trapping

Individual yeast cells can be successfully isolated and recultured on plates with a new isolation method making use of optical trapping with infrared laser light. The cells can be selected on morphological criteria by high resolution microscopy. The isolation device is constructed from two coverslips separated by spacers, in which selected cells are transferred to a plastic capillary, using the optical trap. To test the procedure, selection experiments were done with a mixture of two Saccharomyces cerevisiae strains, distinguishable both in fluorescence microscopy and on agar plates. These experiments showed that only selected cells were isolated, and close to 100% of the isolated stationary-phase cells formed colonies on agar plates, indicating a high recovery. A lower recovery was obtained with exponential-phase cells, possibly because of a higher sensitivity to laser irradiation. Applications for this method may include the isolation of mutants with altered morphology and the isolation of subpopulations of yeast cultures, for their separate investigation or for the initiation of pure cultures.     

Optimal and Suboptimal Quadriphase Sequences Derived from Maximal Length Sequences over Z _{{\bf 4}}

The paper presents families of quadriphase sequences derived from maximal length sequences over having good correlation properties. These are: (i) families of quadriphase sequences of period from maximal length sequences over ; each family consisting of sequences, (ii) families of quadriphase sequences of period from interleaved maximal length sequences over ; each family consisting of sequences. Such sequences are of interest in quadriphase modulated code division multiple access communication systems, where it is desirable to have large sets of sequences that possess low value of , the maximum magnitude of the periodic crosscorrelation and out of phase auto-correlation values. The sequences over are viewed as trace functions of appropriately chosen unit elements of Galois extension rings of . Quadriphase sequences are then obtained from sequences, by a quadriphase mapping, , from to roots of unity, given by, ; where and . Periodic correlation properties (correlation values and their distribution) of the quadriphase sequences are obtained by using an Abelian association scheme on the elements of the corresponding Galois extension ring of . The majority of the families of sequences derived are optimal with respect to the Welch lower bound on ; the rest being suboptimal with bounded by , where is the period\newpage of the sequences. However nearly half of the sequences in these families are balanced. Received July 18, 1995; revised version November 29, 1996     

Stress concentration and fatigue of profiled reinforcing steels

Stress concentrations arise from profiles of ribbed reinforcing steel bars and in this study the results of calculated stress concentration factors (SCF), by using finite element method, are related to the fatigue test results. It is apparent that the degree of confinement of a ribbed bar embedded in concrete not only affects the magnitude of the ultimate bond stress but also the bond stress-slip relation. Thus the rib geometry or profile pattern is designed for optimum bond characteristics and not for fatigue considerations. However the rib geometry influences the fatigue performance through the SCFs arising from the root radius, width and flank angles of the profiles. It is shown that these latter factors have a significant influence on the fatigue behaviour of reinforcing steel bars.     

A calibration method for the energy-integrating function in photovoltaic system monitoring

Solar radiation has an irregularly varying factor due to a basic day and night cycle and climatic conditions. For such conditions a data sampling interval is important to ensure the accuracy of energy monitoring in a photovoltaic system. While treating a system monitoring equipment as a black box. the author has developed the method of calibrating an energy-integrating function. At first for various input waveforms, the relationship between sampling intervals and quasi-integration outputs have been examined by trapezoidal rule. In the numerical simulation the phases of the sampling clock also are considered Then it is concluded that a sampling interval can be inspected through outside observation only by using a rectangular single pulse. By applying the pulse to the energy-integrating process, two kinds of integrated outputs can be obtained for different sampling phases. The calculated difference between both outputs can uniquely give the sampling interval being inspected. Conditions to ensure measuring accuracy are discussed and the validity of this method has been demonstrated experimentally. Practical calibrating procedures also are proposed for the integrating function of PV system monitoring.     

Prediction of the elastic behaviour of hybrid and non-hybrid woven composites

A micromechanical model called MESOTEX is presented for prediction of the elastic behaviour of composites reinforced with non-hybrid weave (plain weave, satin weave and twill weave) and hybrid weave (hybrid plain weave and hybrid twill weave) fabrics. By using the classical thin laminate theory applied to each woven structure, this analytical model takes into account the strand undulations in the two directions and also integrates the geometrical and mechanical parameters of each constituent (resin, fill and warp strands). A representative volume is chosen for each woven composite and the fibre architecture is described by several functions. To determine the effectiveness of this analysis, the elastic properties predicted for each woven composite are compared with experimental results and results extracted from the literature. This correlation shows excellent agreement between measured and predicted values, with a very low calculation cost (CPU time of less than 0·01 s).     

伤寒沙门氏菌特异性θ诊断血清的应用

应用新的特异性θ诊断血清和传统商品血清与434株伤寒沙门氏菌地方株作玻片凝集平行试验，两者诊断符合率为100％。θ血清与0．1％酚琼脂斜面上的所有伤寒沙门氏菌呈迅速清晰的阳性凝集反应。鉴于θ血清与大多数D群沙门氏菌不凝集，因此，大大提高诊断的正确性。     

一种气体缓流流量测量用传感器的研究

本文提出了一种采用悬挂靶的流量传感器，并对其工作特性进行了理论分析及试验研究，这种传感器具有很好的低流速（流量）测量特性，并且具有易于交换量程，工作可靠等优点，试验表明，悬挂靶式流量传感器可测量流速低于１ｍ／ｓ的空气管流。     

低空大气对连续波干涉仪系统相位稳定性的影响

针对三站连续波干涉仪系统，根据实时测得的系统场区大气折射率漂移数据，应用几何光学方法，从理论上估计了连续波干波仪系统两条微波基线传输的相位稳定性。     

小波变换在手写体汉字识别中的应用

汉字识别中的结构类特征具有抗变形的优点，合适做为手写体识别过程使用，但其中笔划特征的提取非常困难。本文用小波变换这一图象分析中有力工具对汉字图象分析处理之后，再进行笔划的提取，其过程简洁、明晰。     

基于Matlab的线性规划问题求解

线性规划是运筹学中研究较早、发展较快、应用广泛、方法成熟的一个重要分支,它是辅助人们进行科学管理的一种重要的数学方法.文章首先介绍了线性规划的基本概念及标准形式,着重讨论了线性规划问题的三种常用解法:单纯形法、直接搜索法以及遗传算法,最后在Matlab R2009a环境下进行了仿真.通过结果可以看出,用Matlab求解线性规划问题,可以避免手工的烦琐计算,大大地提高工作效率和结果的准确性.